HEME oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed1) Lovenberg, T.W., et al., (2005) Biochem. Biophys. Res. Commun. 338 (1), 648-652.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMOX2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HMOX2 antibody can be used for detection of HMOX2 by ELISA at 1:312500. HMOX2 antibody can be used for detection of HMOX2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
HMOX2 antibody can be used for detection of HMOX2 by ELISA at 1:312500. HMOX2 antibody can be used for detection of HMOX2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMOX2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.