Application
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPA0 ppt. k562 sample ).Application
Antibody used in IF on MCF7 cells at 1:200.Application
Antibody used in WB on Human Jurkat 0.625 ug/ml.Application
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate ).Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.| Product Name | HNRPA0 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRPA0, HNRPA0 |
| Product description | HNRPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.1) Lai, F., (2001) Genomics 71 (2), 235-245. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNRPA0. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| HNRPA0 antibody can be used for detection of HNRPA0 by ELISA at 1:312500. HNRPA0 antibody can be used for detection of HNRPA0 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HNRNPA0 |
|---|---|
| Alternative Names | HNRPA0 HNRPA0 |
| Molecular Weight(MW) | 34 kDa |
Application
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPA0 ppt. k562 sample ).
Application
Antibody used in IF on MCF7 cells at 1:200.
Application
Antibody used in WB on Human Jurkat 0.625 ug/ml.
Application
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate ).
Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HNRPA0 antibody can be used for detection of HNRPA0 by ELISA at 1:312500. HNRPA0 antibody can be used for detection of HNRPA0 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HNRPA0 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
