Application
Western blot analysis of HOMER3 using rabbit polyclonal HOMER3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.Application
Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HOMER3 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue with HOMER3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HOMER3 Antibody in MDA-MB231 cell line lysates (35ug/lane)| Product Name | HOMER3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homer protein homolog 3, Homer-3, HOMER3 |
| Product description | HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. This protein binds numerous other proteins including group I metabotropic glutamate receptors,inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.1) Norton,N., et.al., Am. J. Med. Genet.Neuropsychiatr. Genet. 120B (1), 18-21 (2003) |
| Immunogen | This HOMER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-308 amino acids from the Central region of human HOMER3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOMER3 |
|---|---|
| Alternative Names | Homer protein homolog 3 Homer-3 HOMER3 |
| Molecular Weight(MW) | 40 kDa |
Application
Western blot analysis of HOMER3 using rabbit polyclonal HOMER3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.
Application
Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HOMER3 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue with HOMER3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HOMER3 Antibody in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
