Application
Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.Application
Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.Application
Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.| Product Name | HOOK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hook microtubule-tethering protein 3, HK3, h-hook3, hHK3, FLJ31058, Protein Hook homolog 3 |
| Product description | The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).1) Walenta JH, Didier AJ, Liu X, et al. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 2001; 152:923-34. |
| Immunogen | HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3. The immunogen is located within amino acids 210 - 260 of HOOK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOOK3 |
|---|---|
| Alternative Names | hook microtubule-tethering protein 3 HK3 h-hook3 hHK3 FLJ31058 Protein Hook homolog 3 |
| Molecular Weight(MW) | Predicted: 79 kDa Observed: 77 kDa |
| Tissue Specificity | HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2. |
Application
Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.
Application
Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.
Application
Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | HOOK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide. |
