Hoxd3 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.1) Bedford, M., (1995) DNA Cell Biol. 14 (4), 295-304.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse HOXD3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HOXD3 antibody can be used for detection of HOXD3 by ELISA at 1:2500. HOXD3 antibody can be used for detection of HOXD3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
HOXD3 antibody can be used for detection of HOXD3 by ELISA at 1:2500. HOXD3 antibody can be used for detection of HOXD3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HOXD3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.