Hoxd3 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.1) Patterson, L.T. (2004) Dev. Dyn. 229 (4), 771-779.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse HOXD3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HOXD3 antibody can be used for detection of HOXD3 by ELISA at 1:62500. HOXD3 antibody can be used for detection of HOXD3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Spinal cord at 0.2-1 ug/ml.
Positive Control
1) Spinal Cord Lysate using HOXD3
Application Notes
HOXD3 antibody can be used for detection of HOXD3 by ELISA at 1:62500. HOXD3 antibody can be used for detection of HOXD3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HOXD3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.