Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with HP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HP using rabbit polyclonal HP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HP gene (Lane 2).Application
Western Blot at 1:8000 dilution + Human liver lysate Lysates/proteins at 20 ug per lane.| Product Name | HP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Haptoglobin, Zonulin, Haptoglobin alpha chain, Haptoglobin beta chain, HP |
| Product description | HP is a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia.1) Ryndel,M., et.al., Clin. Chim. Acta 411 (7-8), 500-504 (2010) Igl,W., PLoS Genet.(1), E1000798 (2010) |
| Immunogen | This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-322 amino acids from the Central region of human HP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HP |
|---|---|
| Alternative Names | Haptoglobin Zonulin Haptoglobin alpha chain Haptoglobin beta chain HP |
| Molecular Weight(MW) | 45 kDa |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with HP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HP using rabbit polyclonal HP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HP gene (Lane 2).
Application
Western Blot at 1:8000 dilution + Human liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
