Application
Flow cytometric analysis of HepG2 cells using HPD Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HPD Antibody in mouse liver tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HPD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD |
| Product description | HPD is an enzyme in the catabolic pathway of tyrosine. This protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate.1) Item,C.B., et.al., Mol. Genet. Metab. 91 (4), 379-383 (2007) |
| Immunogen | This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human HPD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HPD |
|---|---|
| Alternative Names | 4-hydroxyphenylpyruvate dioxygenase 4-hydroxyphenylpyruvic acid oxidase 4HPPD HPD HPPDase HPD PPD |
| Molecular Weight(MW) | 45 kDa |
Application
Flow cytometric analysis of HepG2 cells using HPD Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HPD Antibody in mouse liver tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
