Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HRIHFB2025 Antibody in MDA-MB231 cell line lysates (35ug/lane)| Product Name | HRIHFB2025 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein chibby homolog 1, ARPP-binding protein, Cytosolic leucine-rich protein, PIGEA-14, PKD2 interactor, Golgi and endoplasmic reticulum-associated 1, CBY1, ARB1, C22orf2, CBY, PGEA1 |
| Product description | Inhibits the Wnt/Wingless pathway by binding to beta catenin and inhibiting beta catenin mediated transcriptional activation by competing with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin 2/PKD2 and possibly of other intracellular proteins.1) Gauci S., et.al., Anal. Chem. 81:4493-4501(2009). |
| Immunogen | This HRIHFB2025 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the C-terminal region of human HRIHFB2025. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBY1 |
|---|---|
| Alternative Names | Protein chibby homolog 1 ARPP-binding protein Cytosolic leucine-rich protein PIGEA-14 PKD2 interactor Golgi and endoplasmic reticulum-associated 1 CBY1 ARB1 C22orf2 CBY PGEA1 |
| Molecular Weight(MW) | 14 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HRIHFB2025 Antibody in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
