Application
Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.| Product Name | Hrk Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hrk Antibody: DP5, HARAKIRI, BID3, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3 |
| Product description | Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk. The immunogen is located within the last 50 amino acids of Hrk. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HRK |
|---|---|
| Alternative Names | Hrk Antibody: DP5 HARAKIRI BID3 Activator of apoptosis harakiri BH3-interacting domain-containing protein 3 |
| Molecular Weight(MW) | Predicted: 10 kDa Observed: 12 kDa |
Application
Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1414 - Mouse Pancreas Tissue Lysate |
|---|---|
| Application Notes | Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Hrk antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Hrk Antibody is supplied in PBS containing 0.02% sodium azide. |
