Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS3ST2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HS3ST2 Antibody in Hela, 293 cell line lysates (35ug/lane)| Product Name | HS3ST2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heparan sulfate glucosamine 3-O-sulfotransferase 2, Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2, 3-OST-2, Heparan sulfate 3-O-sulfotransferase 2, h3-OST-2, HS3ST2, 3OST2 |
| Product description | Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity.1) Ikeda, M., et al. Biol. Psychiatry 67(3):263-269(2010) |
| Immunogen | This HS3ST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human HS3ST2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HS3ST2 |
|---|---|
| Alternative Names | Heparan sulfate glucosamine 3-O-sulfotransferase 2 Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2 3-OST-2 Heparan sulfate 3-O-sulfotransferase 2 h3-OST-2 HS3ST2 3OST2 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HS3ST2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HS3ST2 Antibody in Hela, 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
