17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics.1) Lukacik, P., (2007) Biochem. J. 402 (3), 419-427.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSD17B14.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HSD17B14 antibody can be used for detection of HSD17B14 by ELISA at 1:1562500. HSD17B14 antibody can be used for detection of HSD17B14 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
HSD17B14 antibody can be used for detection of HSD17B14 by ELISA at 1:1562500. HSD17B14 antibody can be used for detection of HSD17B14 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HSD17B14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.