Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
HSD17B2 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HSD17B2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Estradiol 17-beta-dehydrogenase 2, 17-beta-hydroxysteroid dehydrogenase type 2, 17-beta-HSD 2, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Microsomal 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase, HSD17B2, EDH17B2 |
| Product description | HSD17B2 is capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone.HSD17B2 also has 20-alpha-HSD activity. HSD17B2 uses NADH while EDH17B3 uses NADPH.1) Shen, Z., et al. Endocrinology 150(11):4941-4949(2009) |
| Immunogen | This HSD17B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human HSD17B2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSD17B2 |
|---|---|
| Alternative Names | Estradiol 17-beta-dehydrogenase 2 17-beta-hydroxysteroid dehydrogenase type 2 17-beta-HSD 2 20 alpha-hydroxysteroid dehydrogenase 20-alpha-HSD E2DH Microsomal 17-beta-hydroxysteroid dehydrogenase Testosterone 17-beta-dehydrogenase HSD17B2 EDH17B2 |
| Molecular Weight(MW) | 43 kDa |
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
HSD17B2 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
