HSD17B6 has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. HSD17B6 is a member of the retinol dehydrogenase family.The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. Transcript variants utilizing alternative polyadenylation signals exist.1) Huang, X.F. (2001) Biochim. Biophys. Acta 1520 (2), 124-130.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSD17B6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
HSD17B6 antibody can be used for detection of HSD17B6 by ELISA at 1:62500. HSD17B6 antibody can be used for detection of HSD17B6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human A172 cells at 1.25 ug/ml.
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Positive Control
1) A172 Cell Lysate
Application Notes
HSD17B6 antibody can be used for detection of HSD17B6 by ELISA at 1:62500. HSD17B6 antibody can be used for detection of HSD17B6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HSD17B6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt