Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | HSPA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA4, APG-2, HS24/P52, MGC131852, RY, hsp70, hsp70RY, HSPH2, HEL-S-5a |
| Product description | HSPA4 (Hsp70) belongs to the heat shock protein 70 family. It was isolated as a putative Rictor interacting protein and interaction with membranes acts as a platform for its release into the extracellular environment during its recovery from stress. Hsp70 gene expression in Rheumatoid Arthitis-affected synovial tissue is followed by Hsp70 cell surface expression on fibroblast-like synovial cells growing from RA synovial tissue. Serum Hsp70 levels were increased in Systemic sclerosis patients, and associated with pulmonary fibrosis, skin sclerosis, renal vascular damage, oxidative stress, and inflammation.1) Kim, H.E., (2008) Mol. Cell 30 (2), 239-247. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSPA4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HSPA4 antibody can be used for detection of HSPA4 by ELISA at 1:62500. HSPA4 antibody can be used for detection of HSPA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPA4 |
|---|---|
| Alternative Names | HSPA4 APG-2 HS24/P52 MGC131852 RY hsp70 hsp70RY HSPH2 HEL-S-5a |
| Molecular Weight(MW) | 94 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | HSPA4 antibody can be used for detection of HSPA4 by ELISA at 1:62500. HSPA4 antibody can be used for detection of HSPA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HSPA4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
