IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.ICC/IF
Immunofluorescence analysis of NIH3T3 cells using HSP70 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.IHC
Immunohistochemical analysis of paraffin-embedded Human muscle tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-HSP70 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC |
| IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSPA4 |
|---|---|
| Gene Synonyms | 70kDa 87A APG-2 Bb CELE_C12C8.1 CG31359 CG31366 dHSP70 dhsp70Aa Dm-hsp70 Dmel\CG31359 Dmel\CG31366 Dmel_CG31359 Dmel_CG31366 DmHSP70AA DMHSP7D1 HEL-S-103 HEL-S-5a HS24/P52 hs70 Hsc70t HSP 70 Hsp-70 Hsp-70Aa Hsp110 hsp68 HSP70 hsp70 87A hsp70 87C hsp70 Aa hsp70(87A) Hsp70(87C) HSP70-1 HSP70-1A HSP70-1B HSP70-1L HSP70-2 HSP70-3 HSP70-HOM HSP70.1 HSP70.2 Hsp70.3 Hsp70A hsp70A1 HSP70A2 Hsp70A7d hsp70Aa Hsp70B HSP70B1 Hsp70bb Hsp70Bbc Hsp70Bc HSP70I Hsp70RY HSP70T HSP72 HSPA1 HSPA1A HSPA1B Hspa1l HSPA2 HSPA3 HSPH2 Hspt70 Hst70 HSX70 hum70t I79_004332 irp94 mKIAA4025 Msh5 RY |
| Gene Full Name | heat shock protein family A (Hsp70) member 4 |
| Gene Summary | Predicted to enable ATP binding activity. Involved in chaperone-mediated protein complex assembly and protein insertion into mitochondrial outer membrane. Located in cytosol and extracellular exosome. Implicated in Chagas disease. Biomarker of chronic obstructive pulmonary disease; rheumatoid arthritis; type 2 diabetes mellitus; and ulcerative colitis. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | 68 kDa heat shock protein 70 kda heat shock protein-2 70 kilodalton heat shock protein 87A locus 87C locus ARABIDOPSIS HEAT SHOCK PROTEIN 70 ATHSP70 CG31359-PA CG31366-PA chaperone dnaK-type molecular chaperone HSP70-1 epididymis secretory protein Li 103 epididymis secretory sperm binding protein epididymis secretory sperm binding protein Li 5a heat shock 10kDa protein 1-like heat shock 70 heat shock 70 kD protein 1 heat shock 70 kD protein 2 heat shock 70 kD protein 3 Heat shock 70 kDa protein 1 heat shock 70 kDa protein 1-Hom heat shock 70 kDa protein 1/2 Heat shock 70 kDa protein 1A Heat shock 70 kDa protein 1A/1B Heat shock 70 kDa protein 1B Heat shock 70 kDa protein 1L Heat shock 70 kDa protein 2 heat shock 70 kDa protein 3 Heat shock 70 kDa protein 4 heat shock 70 kDa-like protein 1 heat shock 70-related protein APG-2 heat shock 70kD protein 1A heat shock 70kD protein 1B heat shock 70kD protein 2 heat shock 70kD protein 4 heat shock 70kD protein-like 1 heat shock 70kDa protein 1-like heat shock 70kDa protein 1A heat shock 70kDa protein 1B heat shock 70kDa protein 2 heat shock 70kDa protein 3 heat shock 70kDa protein 4 Heat shock protein heat shock protein 1-like heat shock protein 1A heat shock protein 1B heat shock protein 2 heat shock protein 4 Heat Shock Protein 70 heat shock protein 70-1 Heat shock protein 70-1l heat shock protein 70.1 heat shock protein 70.2 heat shock protein alpha 2 heat shock protein Apg-2 heat shock protein cognate 70 testis heat shock protein family A (Hsp70) member 1 like heat shock protein family A member 1A heat shock protein family A member 1B heat shock protein family A member 1L heat shock protein family A member 2 heat shock protein family A member 4 heat shock protein family H member 2 heat shock protein 110 kDa heat shock protein 70 kDa 1 heat shock protein 70 kDa 2 heat shock protein 70 kDa 3 heat shock protein-70 heat shock-induced protein Heat shock-related 70 kDa protein 2 heat shock-related protein heat shock-related protein hsc70t heat-shock 70-kilodalton protein 1A heat-shock 70-kilodalton protein 1B heat-shock protein 70 HEL S 103 HSP HSP70 1 HSP70 1B HSP70 2 hsp70 87A HSP70 AceyltK77 hsp70 RY HSP70-1/HSP70-2 HSP70.1 HSP70.1/2 HSP70.1/HSP70.2 HSP70.2 HSP70.3 Hsp70Aa-PA Hsp70Bb-PA HSPA2 HSPA4 Hspa70 HST inducible heat shock protein 70 IRP94 ischemia responsive 94 kDa protein LP05203p LP05233p LP08776p protein 70 spermatid-specific heat shock protein 70 testis-specific heat shock protein-related gene hst70 |
| Molecular Weight(MW) | 94KD |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P34932 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using HSP70 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
IHC
Immunohistochemical analysis of paraffin-embedded Human muscle tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HSP70 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
