Application
Western blot analysis in A431,H-4-II-E,Hela,rat L6 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSPCB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HSPCB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB |
| Product description | HSPCB proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSPCB proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.1) Chadli,A., et.al., J. Biol. Chem. 285 (6), 4224-4231 (2010) |
| Immunogen | This HSPCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-74 amino acids from the N-terminal region of human HSPCB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HSP90AB1 |
|---|---|
| Alternative Names | Heat shock protein HSP 90-beta HSP 90 Heat shock 84 kDa HSP 84 HSP84 HSP90AB1 HSP90B HSPC2 HSPCB |
| Molecular Weight(MW) | 83 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A431,H-4-II-E,Hela,rat L6 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with HSPCB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
