This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibits adenylate cyclase activity.1) Czesak, M., (2006) J. Neurosci. 26 (6), 1864-1871.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HTR1A.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HTR1A antibody can be used for detection of HTR1A by ELISA at 1:62500. HTR1A antibody can be used for detection of HTR1A by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Jurkat 1.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human myometrial.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
HTR1A antibody can be used for detection of HTR1A by ELISA at 1:62500. HTR1A antibody can be used for detection of HTR1A by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HTR1A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.