WB
Western blot analysis of extracts from 293, using 5-HT-1A Antibody. The lane on the left was treated with blocking peptide.WB
Western blot analysis of extracts from various samples, using 5HT1A Receptor Antibody. Lane 1: Hepg2 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Hepg2 cells(serum starvation treatment). Lane 3: Hela cells(heat-shock treatment).IHC
5HT1A Receptor Antibody at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
5HT1A Receptor Antibody staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to 5HT1A Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human 5HT1A Receptor, corresponding to a region within the internal amino acids.. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HTR1A |
|---|---|
| Gene Synonyms | G-21 5HT1a PFMCD 5-HT1A 5-HT-1A ADRBRL1 ADRB2RL1 |
| Gene Full Name | 5-hydroxytryptamine receptor 1A |
| Gene Summary | This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012] |
| Alternative Names | 5 HT 1A 5 HT receptor 1A 5 HT1A 5 HT1A receptor 5 hydroxytryptamine (serotonin) receptor 1A 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled 5 hydroxytryptamine receptor 1A 5-HT-1A 5-HT1A 5-hydroxytryptamine receptor 1A 5HT 1A 5HT 1A receptor 5HT receptor 1A 5HT1a 5HT1A_HUMAN ADRB2RL1 ADRBRL1 G 21 G protein coupled receptor G-21 Gpcr 18 Gpcr18 Guanine nucleotide binding regulatory protein-coupled receptor Htr1a PFMCD Serotonin 1A receptor Serotonin 5HT1A receptor Serotonin receptor 1A |
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cell membrane>Multi-pass membrane protein. |
WB
Western blot analysis of extracts from 293, using 5-HT-1A Antibody. The lane on the left was treated with blocking peptide.
WB
Western blot analysis of extracts from various samples, using 5HT1A Receptor Antibody. Lane 1: Hepg2 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Hepg2 cells(serum starvation treatment). Lane 3: Hela cells(heat-shock treatment).
IHC
5HT1A Receptor Antibody at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
5HT1A Receptor Antibody staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
