Application
Confocal immunofluorescent analysis of HtrA1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
HtrA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.| Product Name | HtrA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine protease HTRA1, 3421-, High-temperature requirement A serine peptidase 1, L56, Serine protease 11, HTRA1, HTRA, PRSS11 |
| Product description | HtrA1 a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.1) Howes, N., et al., Clin Gastroenterol Hepatol 2(3):252-261 (2004). |
| Immunogen | This HtrA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-147 amino acids from the N-terminal region of human HtrA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | HTRA1 |
|---|---|
| Alternative Names | Serine protease HTRA1 3421- High-temperature requirement A serine peptidase 1 L56 Serine protease 11 HTRA1 HTRA PRSS11 |
| Molecular Weight(MW) | 51 kDa |
Application
Confocal immunofluorescent analysis of HtrA1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
HtrA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
