Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect HUNK in NIH/3T3 cell lysate.| Product Name | HUNK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-V, HUNK, MAKV |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.1) Gardner, H.P., et al., Genomics 63(1):46-59 (2000). |
| Immunogen | This HUNK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 659-690 amino acids from the C-terminal region of human HUNK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | HUNK |
|---|---|
| Alternative Names | Hormonally up-regulated neu tumor-associated kinase B19 Serine/threonine-protein kinase MAK-V HUNK MAKV |
| Molecular Weight(MW) | 80 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect HUNK in NIH/3T3 cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
