Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | HUS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Checkpoint protein HUS1, hHUS1, HUS1 |
| Product description | The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. [provided by RefSeq].1) Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010) |
| Immunogen | This HUS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-226 amino acids from the C-terminal region of human HUS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HUS1 |
|---|---|
| Alternative Names | Checkpoint protein HUS1 hHUS1 HUS1 |
| Molecular Weight(MW) | 32 kDa |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
