Application
Immunohistochemistry of HVCN1 in rat spleen tissue with HVCN1 antibody at 5 μg/mL.Application
Immunofluorescence of HVCN1 in Rat Spleen cells with HVCN1 antibody at 20 μg/mL.Application
Western blot analysis of HVCN1 in (A) human spleen and (B) mouse spleen tissue lysates with HVCN1 antibody at 1 μg/mL.| Product Name | HVCN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HVCN1 Antibody: HV1, VSOP, UNQ578/PRO1140, Voltage-gated hydrogen channel 1, Hydrogen voltage-gated channel 1, HV1 |
| Product description | HVCN1 Antibody: HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.1) Ramsey IS, Moran MM, Chong JA, et al. A voltage gated proton-selective channel lacking the pore domain. Nature2006; 440:1213-6. |
| Immunogen | HVCN1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human HVCN1. The immunogen is located within the last 50 amino acids of HVCN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HVCN1 antibody can be used for detection of HVCN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HVCN1 |
|---|---|
| Alternative Names | HVCN1 Antibody: HV1 VSOP UNQ578/PRO1140 Voltage-gated hydrogen channel 1 Hydrogen voltage-gated channel 1 HV1 |
| Molecular Weight(MW) | Predicted: 30 kDa Observed: 30 kDa |
| Sequence Similarities | 2) Cat. No. 1306 - Human Spleen Tissue Lysate |
Application
Immunohistochemistry of HVCN1 in rat spleen tissue with HVCN1 antibody at 5 μg/mL.
Application
Immunofluorescence of HVCN1 in Rat Spleen cells with HVCN1 antibody at 20 μg/mL.
Application
Western blot analysis of HVCN1 in (A) human spleen and (B) mouse spleen tissue lysates with HVCN1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
|---|---|
| Application Notes | HVCN1 antibody can be used for detection of HVCN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | HVCN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HVCN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
