Application
Western blot analysis of IA-1 in rat thymus tissue lysate with IA-1 antibody at 1 μg/mL.| Product Name | IA-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IA-1 Antibody: IA1, IA-1, IA1, Insulinoma-associated protein 1, Zinc finger protein IA-1 |
| Product description | IA-1 Antibody: IA-1, also known as INSM1, is an essential five zinc-finger transcription factor that while initially identified from an insulinoma cDNA library, is expressed in the developing nervous system. Specifically, IA-1 is expressed as early as E9.5 in mice in the fore-, mid- and hindbrain, spinal cord, retina, and olfactory bulb. It is thought that IA-1 functions as a transcriptional repressor, and is a part of several signaling pathways including those of Notch and sonic hedgehog in addition to that of Ngn3 during pancreatic endocrine cell differentiation. IA-1 is also expressed in multiple tumors, including the majority of neuroendocrine tumors tested so far as well as nearly 100% of all small cell lung carcinomas, indicating that IA-1 may be an important target in cancer therapy.1) Lan MS, Li Q, Lu J, et al. Genomic organization, 5’-upstream sequence, and chromosomal localization of an insulinoma-associated intronless gene, IA-1. J. Biol. Chem.1994; 269:14170-4. |
| Immunogen | IA-1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human IA-1. The immunogen is located within amino acids 90 - 140 of IA-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IA-1 antibody can be used for detection of IA-1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INSM1 |
|---|---|
| Alternative Names | IA-1 Antibody: IA1 IA-1 IA1 Insulinoma-associated protein 1 Zinc finger protein IA-1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Western blot analysis of IA-1 in rat thymus tissue lysate with IA-1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1471 - Rat Thymus Tissue Lysate |
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| Application Notes | IA-1 antibody can be used for detection of IA-1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IA-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IA-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
