Application
Immunohistochemistry of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.Application
Immunofluorescence of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.Application
Western blot analysis of ICAD in mouse lung (L), brain (B), liver (L), and kidney tissue lysate with CAD antibody at 1:1000 dilution.| Product Name | ICAD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45 |
| Product description | ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.1) Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 |
| Immunogen | ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD. The immunogen is located within the last 50 amino acids of ICAD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Dffa |
|---|---|
| Alternative Names | ICAD Antibody: ICAD DFF35 Dff45 ICAD-L ICAD-S A330085O09Rik Icad DNA fragmentation factor subunit alpha DNA fragmentation factor 45 kDa subunit DFF-45 |
| Molecular Weight(MW) | 45 kDa |
Application
Immunohistochemistry of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.
Application
Immunofluorescence of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.
Application
Western blot analysis of ICAD in mouse lung (L), brain (B), liver (L), and kidney tissue lysate with CAD antibody at 1:1000 dilution.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ICAD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ICAD Antibody is supplied in PBS containing 0.02% sodium azide. |
