Application
Western blot analysis in Hela,Jurkat,U251,MDA-MB453,NCI-H292,K562,ZR-75-1 cell line lysates (35ug/lane).Application
Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Product Name | ICT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Peptidyl-tRNA hydrolase ICT1, mitochondrial, 39S ribosomal protein L58, mitochondrial, MRP-L58, Digestion substraction 1, DS-1, Immature colon carcinoma transcript 1 protein, ICT1, DS1 |
| Product description | Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. |
| Immunogen | This ICT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-179 amino acids from the C-terminal region of human ICT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ICT1 |
|---|---|
| Alternative Names | Peptidyl-tRNA hydrolase ICT1 mitochondrial 39S ribosomal protein L58 mitochondrial MRP-L58 Digestion substraction 1 DS-1 Immature colon carcinoma transcript 1 protein ICT1 DS1 |
| Molecular Weight(MW) | 24 kDa |
Application
Western blot analysis in Hela,Jurkat,U251,MDA-MB453,NCI-H292,K562,ZR-75-1 cell line lysates (35ug/lane).
Application
Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
