Application
Western blot analysis of lysate from K562 cell line, using IDE Antibody at 1:1000.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-IDE Antibody in A375 cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.| Product Name | IDE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE |
| Product description | IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.1) Vepsalainen,S.,J. Med. Genet. 44 (9), 606-608 (2007) |
| Immunogen | This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | IDE |
|---|---|
| Alternative Names | Insulin-degrading enzyme Abeta-degrading protease Insulin protease Insulinase Insulysin IDE |
| Molecular Weight(MW) | 118 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of lysate from K562 cell line, using IDE Antibody at 1:1000.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-IDE Antibody in A375 cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
