Application
Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 μg/mL.Application
Immunofluorescence of IDH2 in mouse heart tissue with IDH2 antibody at 20 μg/mL.Application
Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IDH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDHICD-M, IDH |
| Product description | IDH2 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. Both IDH1 and IDH2 play significant roles in the tricarboxylic acid cycle (TCA), and defects in IDH1 as well as IDH2 have been implicated in the development of glioma as well as other malignancies.1) Geisbrecht BV and Gould SJ. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J. Biol. Chem.1999; 274:30527-33. |
| Immunogen | IDH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human IDH2. The immunogen is located within the first 50 amino acids of IDH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IDH2 antibody can be used for detection of IDH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IDH2 |
|---|---|
| Alternative Names | IDH2 Antibody: IDH IDP IDHM IDPM ICD-M D2HGA2 mNADP-IDHICD-M IDH |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (88%) |
Application
Immunohistochemistry of IDH2 in mouse heart tissue with IDH2 antibody at 5 μg/mL.
Application
Immunofluorescence of IDH2 in mouse heart tissue with IDH2 antibody at 20 μg/mL.
Application
Western blot analysis of IDH2 in human heart tissue lysate with IDH2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | IDH2 antibody can be used for detection of IDH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IDH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IDH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
