Application
Antibody used in WB on Rat kidney at 1:1000.Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | IDH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IDH2, ICD-M, IDH, IDHM, IDP, mNADP-IDH, IDPM, D2HGA2 |
| Product description | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. IDH2 is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Shin, S.W., (2008) Biochem. Biophys. Res. Commun. 366 (4), 1012-1018. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IDH2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IDH2 antibody can be used for detection of IDH2 by ELISA at 1:12500. IDH2 antibody can be used for detection of IDH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IDH2 |
|---|---|
| Alternative Names | IDH2 ICD-M IDH IDHM IDP mNADP-IDH IDPM D2HGA2 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Rat kidney at 1:1000.
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | IDH2 antibody can be used for detection of IDH2 by ELISA at 1:12500. IDH2 antibody can be used for detection of IDH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IDH2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
