Application
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using IDH3B Antibody at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded H.brain section using IDH3B Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Product Name | IDH3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, Isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta, IDH3B |
| Product description | 1) Kim Y.-O.,et al.J. Biol. Chem. 274:36866-36875(1999). |
| Immunogen | This IDH3B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human IDH3B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IDH3B |
|---|---|
| Alternative Names | Isocitrate dehydrogenase [NAD] subunit beta mitochondrial Isocitric dehydrogenase subunit beta NAD(+)-specific ICDH subunit beta IDH3B |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using IDH3B Antibody at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded H.brain section using IDH3B Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
