WB
Western blot analysis of IDH3B using anti-IDH3B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LANAP whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IDH3B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a HSP70goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of IDH3B using anti-IDH3B antibody. IDH3B was detected in a paraffin-embedded section of follicles of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-IDH3B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of Jurkat cells using anti-IDH3B antibody. Overlay histogram showing Jurkat cells stained with anti-IDH3B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH3B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.IP
IP analysis of IDH3B using anti-IDH3B antibody in U251 whole cell lysate. Western blot analysis of IDH3B using anti- IDH3B antibody. Lane 1: U251 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- IDH3B antibody in U251 whole cell lysate, Lane 3: anti- IDH3B antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- IDH3B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Anti-IDH3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human IDH3B recombinant protein (Position: A32-D362). Human IDH3B shares 97% amino acid (aa) sequence identity with rat IDH3B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:500-2000 IHC:1:50-400 FC:1:50-200 IP:1:250-300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IDH3B |
|---|---|
| Gene Synonyms | RP46 |
| Gene Full Name | isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta |
| Gene Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2016] |
| Alternative Names | FLJ11043 H IDHB IDH3B RP46 |
| Molecular Weight(MW) | 42kDa(Observed MW 42kDa) |
| Cellular Localization | Mitochondrion |
WB
Western blot analysis of IDH3B using anti-IDH3B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LANAP whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IDH3B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a HSP70goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of IDH3B using anti-IDH3B antibody. IDH3B was detected in a paraffin-embedded section of follicles of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-IDH3B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of Jurkat cells using anti-IDH3B antibody. Overlay histogram showing Jurkat cells stained with anti-IDH3B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH3B Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IP
IP analysis of IDH3B using anti-IDH3B antibody in U251 whole cell lysate. Western blot analysis of IDH3B using anti- IDH3B antibody. Lane 1: U251 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- IDH3B antibody in U251 whole cell lysate, Lane 3: anti- IDH3B antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- IDH3B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-2000 IHC:1:50-400 FC:1:50-200 IP:1:250-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
