Application
Immunohistochemistry of IEX-1 in human liver tissue with IEX-1 antibody at 2.5 μg/mL.Application
Immunofluorescence of IEX-1 in Human Brain cells with IEX-1 antibody at 20 μg/mL.Application
Western blot analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 μg/mL..| Product Name | IEX-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IEX-1 Antibody: DIF2, IEX1, PRG1, DIF-2, GLY96, IEX-1, IEX-1L, DIF2, Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Protein DIF-2 |
| Product description | IEX-1 Antibody: IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-α. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-κB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.1) Kondratyev AD, Chung K-N and Jung MO. Identification and characterization of a radiation-inducible glycosylated human early-response gene. Cancer Res.1996; 56:1498-502. |
| Immunogen | IEX1 antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1. The immunogen is located within amino acids 40 - 90 of IEX-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IEX1 antibody can be used for detection of IEX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IER3 |
|---|---|
| Alternative Names | IEX-1 Antibody: DIF2 IEX1 PRG1 DIF-2 GLY96 IEX-1 IEX-1L DIF2 Radiation-inducible immediate-early gene IEX-1 Differentiation-dependent gene 2 protein Protein DIF-2 |
| Molecular Weight(MW) | Predicted: 17 kDa Observed: 17 kDa |
| Sequence Similarities | 4) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of IEX-1 in human liver tissue with IEX-1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of IEX-1 in Human Brain cells with IEX-1 antibody at 20 μg/mL.
Application
Western blot analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 μg/mL..| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | IEX1 antibody can be used for detection of IEX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IEX-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IEX-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
