Application
Immunohistochemistry of IFIT3 in human liver tissue with IFIT3 antibody at 5 μg/mL.Application
Western blot analysis of IFIT3 in HepG2 cell lysate with IFIT3 antibody at 1 μg/ml.| Product Name | IFIT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFIT3 Antibody : P60, IRG2, IFI60, IFIT4, ISG60, RIG-G, CIG-49, GARG-49, Interferon-induced protein with tetratricopeptide repeats 3, CIG49, IFIT-3 |
| Product description | The interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) protein is a member of a family of tetratricopeptide repeat-containing proteins whose transcription is upregulated by interferons, virus infection, and molecular patterns such as dsRNA or lipopolysaccharides (1,2). These proteins have been suggested to induce anti-viral cellular activities in response to infection (2). IFIT3 has been shown to interact with TBK1 following RNA virus infection, thereby bridging TBK1 to VISA on the mitochondrion and activating IRF3 and thus the immune response (3).1) Yu M, Tong JH, Mao M, et al. Cloning of a gene (RIG-G) associated with retinoic acid-induced differentiation of acute promyelocytic leukemia cells and representing a new member of a family of interferon-stimulating genes. Proc. Natl. Acad. Sci. USA 1997; 94:7406-11. |
| Immunogen | IFIT3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IFIT3. The immunogen is located within amino acids 380 - 430 of IFIT3 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IFIT3 antibody can be used for detection of IFIT3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFIT3 |
|---|---|
| Alternative Names | IFIT3 Antibody : P60 IRG2 IFI60 IFIT4 ISG60 RIG-G CIG-49 GARG-49 Interferon-induced protein with tetratricopeptide repeats 3 CIG49 IFIT-3 |
| Molecular Weight(MW) | Predicted: 54 kDa Observed: 50 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | IFIT3 antibody is human specific. This antibody is predicted to not cross-react with other members of the IFIT protein family. |
| Entrez Gene | 3437 |
|---|---|
| Protein Accession | NP_001026853 |
Application
Immunohistochemistry of IFIT3 in human liver tissue with IFIT3 antibody at 5 μg/mL.
Application
Western blot analysis of IFIT3 in HepG2 cell lysate with IFIT3 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | IFIT3 antibody can be used for detection of IFIT3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IFIT3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IFIT3 Antibody is supplied in PBS containing 0.02% sodium azide. |
