WB
Positive WB detected in: A549 whole cell lysate All lanes: IFIT3 antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IP
Immunoprecipitating IFIT3 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of IFIT3 Antibody in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: IFIT3 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using IFIT3 Antibody at dilution of 1:100ICC/IF
Immunofluorescent analysis of HepG2 cells using IFIT3 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)| Product Name | Rabbit anti-IFIT3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Interferon-induced protein with tetratricopeptide repeats 3 protein (1-490AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:500 IP:1:10-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFIT3 |
|---|---|
| Gene Synonyms | P60 IRG2 IFI60 IFIT4 ISG60 RIG-G cig41 CIG-49 GARG-49 |
| Gene Full Name | interferon induced protein with tetratricopeptide repeats 3 |
| Gene Summary | Enables identical protein binding activity. Involved in antiviral innate immune response; negative regulation of apoptotic process; and negative regulation of cell population proliferation. Located in cytosol and mitochondrion. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CIG 49 antibody CIG49 antibody GARG 49 antibody IFI-60K antibody IFI60 antibody IFI60K antibody IFIT-3 antibody IFIT-4 antibody Ifit3 antibody IFIT3_HUMAN antibody IFIT4 antibody Interferon induced 60 kDa protein antibody Interferon induced protein 60 antibody Interferon induced protein with tetratricopeptide repeats 3 antibody Interferon-induced 60 kDa protein antibody Interferon-induced protein with tetratricopeptide repeats 3 antibody Interferon-induced protein with tetratricopeptide repeats 4 antibody IRG2 antibody ISG-60 antibody ISG60 antibody P60 antibody Retinoic acid induced gene G protein antibody Retinoic acid-induced gene G protein antibody RIG G antibody RIG-G antibody RIGG antibody RP11-149I23.4 antibody |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Cytoplasm. Mitochondrion. |
WB
Positive WB detected in: A549 whole cell lysate All lanes: IFIT3 antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IP
Immunoprecipitating IFIT3 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of IFIT3 Antibody in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: IFIT3 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using IFIT3 Antibody at dilution of 1:100
ICC/IF
Immunofluorescent analysis of HepG2 cells using IFIT3 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)| Application Notes | WB:1:1000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:500 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
