Application
Immunocytochemistry of IFITM1 in Jurkat cells with IFITM1 antibody at 20 μg/mL.Application
Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 μg/mL.Application
Western blot analysis of IFITM1 in 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IFITM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFITM1 Antibody: 9-27, CD225, IFI17, LEU13, DSPA2a, Interferon-induced transmembrane protein 1, Dispanin subfamily A member 2a |
| Product description | IFITM1 Antibody: IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon-gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.1) Deblandre GA, Marinx OP, Evans SS, et al. Expression cloning of an interferon-inducible 17 kDa membrane protein implicated in the control of cell growth. J. Biol. Chem. 1995; 270:23860-6. |
| Immunogen | IFITM1 antibody was raised against a 16 amino acid synthetic peptide near the center of human IFITM1. The immunogen is located within amino acids 40 - 90 of IFITM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IFITM1 antibody can be used for detection of IFITM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFITM1 |
|---|---|
| Alternative Names | IFITM1 Antibody: 9-27 CD225 IFI17 LEU13 DSPA2a Interferon-induced transmembrane protein 1 Dispanin subfamily A member 2a |
| Molecular Weight(MW) | Predicted: 14 kDa Observed: 14 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of IFITM1 in Jurkat cells with IFITM1 antibody at 20 μg/mL.
Application
Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 μg/mL.
Application
Western blot analysis of IFITM1 in 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | IFITM1 antibody can be used for detection of IFITM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IFITM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IFITM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
