Application
Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 5 μg/mL.Application
Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 2.5 μg/ml.Application
Western blot analysis of IFN-b in A-20 cell lysate with IFN-b antibody at 5 μg/mL.Application
Immunofluorescence of IFN-beta in Human Liver cells with IFN-beta antibody at 20 μg/mL.| Product Name | IFN-beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFN-beta Antibody: IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast interferon, IFN-beta |
| Product description | IFN-beta Antibody: Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by "Natural IFN-producing cells" (IPCs) as part of the host immune response and can also inhibit the development of tumors. IFN-beta binding by its receptor results in the activation of the tyrosine kinases Jak1 and Tyk2 and phosphorylation of members of the STAT family of transcription factors, leading to the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.1) Gresser I. Wherefore interferon? J. Leuk. Biol.1997; 61:567-74. |
| Immunogen | IFN-beta antibody was raised against a 17 amino acid synthetic peptide from near the center of human IFN-b. The immunogen is located within amino acids 110 - 160 of IFN-beta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IFN-b antibody can be used for detection of IFN-b by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFNB1 |
|---|---|
| Alternative Names | IFN-beta Antibody: IFB IFF IFNB IFB Interferon beta Fibroblast interferon IFN-beta |
| Sequence Similarities | 3) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 5 μg/mL.
Application
Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 2.5 μg/ml.
Application
Western blot analysis of IFN-b in A-20 cell lysate with IFN-b antibody at 5 μg/mL.
Application
Immunofluorescence of IFN-beta in Human Liver cells with IFN-beta antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | IFN-b antibody can be used for detection of IFN-b by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IFN-beta antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide. |
