Application
To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.Application
To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human IFN-gamma when used with ProSci's biotinylated antigen affinity purified anti-human IFN-gamma (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.| Product Name | IFN-gamma Antibody [1.1-5A9-2D05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The monoclonal antibody was produced in BALB/c x ICR F1 mice using recombinant IFN-gamma as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISA,WB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IFN-γ when used with our biotinylated antigen affinity purified anti-human IFN-γ (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL. Western Blot: To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.0-3.0 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFNG |
|---|---|
| Alternative Names | AF1 Interferon gamma receptor Interferon gamma transducerInterferon gamma Immune interferon IFN-gamma IFG IFI |
Application
To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.
Application
To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.
Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human IFN-gamma when used with ProSci's biotinylated antigen affinity purified anti-human IFN-gamma (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IFN-γ when used with our biotinylated antigen affinity purified anti-human IFN-γ (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL. Western Blot: To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.0-3.0 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IFN-γ lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
