Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
To detect Human IFN-λ2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-λ2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IFN-λ2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IFN-λ2 (38-172) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-λ2.| Product Name | IFN-lamda2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli derived Recombinant Human IFN-λ2 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,IHC,WB |
| ELISA: Sandwich: To detect Human IFN-lamda2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IFN-lamda2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-lamda2. Western Blot: : To detect Human IFN-lamda2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-lamda2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/mL overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFNL2 |
|---|---|
| Alternative Names | IL28A IL-28A IL28A ZCYTO20 Interferon lambda-2 Cytokine Zcyto20 IFN-lambda-2 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
To detect Human IFN-λ2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-λ2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IFN-λ2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IFN-λ2 (38-172) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-λ2.| Application Notes | ELISA: Sandwich: To detect Human IFN-lamda2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IFN-lamda2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-lamda2. Western Blot: : To detect Human IFN-lamda2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-lamda2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/mL overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
