Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.125 ug/ml- 0.200 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIGF-BP1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.| Product Name | IGF-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AFBP, IBP1, PP12, IGF-BP25, hIGFBP-1, Insulin-like growth factor-binding protein 1, Placental protein 12, IBP-1 |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Human IGF-BP1 specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP1 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: To detect hIGF-BP1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1. Western Blot: To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IGFBP1 |
|---|---|
| Alternative Names | AFBP IBP1 PP12 IGF-BP25 hIGFBP-1 Insulin-like growth factor-binding protein 1 Placental protein 12 IBP-1 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.125 ug/ml- 0.200 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIGF-BP1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.| Application Notes | ELISA: Sandwich: To detect hIGF-BP1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1. Western Blot: To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
