Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentration ranges from 0.5 ug/ml-5.0ug/ml. An HRP-labeled polymer detection system was used with DAB chromogen. Recommended antigen retrievals are heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with Proteinase K. Optimal concentrationApplication
To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.| Product Name | IGF-II Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IGF-II, PP9974, C11orf43Insulin-like growth factor II, Somatomedin-A, IGF-II |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-II (human Insulin Like Growth Factor-II). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1 4.6 mg/mL of this antibody is required. ELISA: To detect hIGF-II by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF2 |
|---|---|
| Alternative Names | IGF-II PP9974 C11orf43Insulin-like growth factor II Somatomedin-A IGF-II |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentration ranges from 0.5 ug/ml-5.0ug/ml. An HRP-labeled polymer detection system was used with DAB chromogen. Recommended antigen retrievals are heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with Proteinase K. Optimal concentration
Application
To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIGF-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1 4.6 mg/mL of this antibody is required. ELISA: To detect hIGF-II by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-II. Sandwich: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IGF-II antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
