Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demodetected the IGF2 protein (arrow).Application
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.Application
IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry.Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | IGF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-like growth factor II, IGF-II, Somatomedin-A, Insulin-like growth factor II, Insulin-like growth factor II Ala-25 Del, Preptin, IGF2 |
| Product description | This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Adkins, R.M., et al. Pediatr. Res. 68(5):429-434(2010) |
| Immunogen | This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF2 |
|---|---|
| Alternative Names | Insulin-like growth factor II IGF-II Somatomedin-A Insulin-like growth factor II Insulin-like growth factor II Ala-25 Del Preptin IGF2 |
| Molecular Weight(MW) | 20 kDa |
Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demodetected the IGF2 protein (arrow).
Application
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.
Application
IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry.
Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
