Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: RD whole cell lysate Lane 3: Karpas 299 whole cell lysate Lane 4: Raji whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HepG2 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Product Name | IGF2BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, CRD-BP, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, ZBP-1, IGF2BP1, CRDBP, VICKZ1 |
| Product description | IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.1) Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010) |
| Immunogen | This IGF2BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-534 amino acids from the C-terminal region of human IGF2BP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF2BP1 |
|---|---|
| Alternative Names | Insulin-like growth factor 2 mRNA-binding protein 1 IGF2 mRNA-binding protein 1 IMP-1 IMP1 Coding region determinant-binding protein CRD-BP IGF-II mRNA-binding protein 1 VICKZ family member 1 Zipcode-binding protein 1 ZBP-1 IGF2BP1 CRDBP VICKZ1 |
| Molecular Weight(MW) | 63 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: RD whole cell lysate Lane 3: Karpas 299 whole cell lysate Lane 4: Raji whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HepG2 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
