WB
Western blot analysis of IGF2BP1 on different lysates with Rabbit anti-IGF2BP1 antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: Caco-2 cell lysate, Lane 3: K-562 cell lysate, Lane 4: 293T cell lysate, Lane 5: MCF7 cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Caco-2 (positive) and MCF7 (negative) labeling IGF2BP1 with Rabbit anti-IGF2BP1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGF2BP1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of MCF7 (left, negative) and Caco-2 (right, positive) cells labeling IGF2BP1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | IGF2BP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human IGF2BP1 aa 528-577. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:2000 ICC/IF:1:100 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IGF2BP1 |
|---|---|
| Gene Synonyms | IMP1 ZBP1 CRDBP IMP-1 CRD-BP VICKZ1 |
| Gene Full Name | insulin like growth factor 2 mRNA binding protein 1 |
| Gene Summary | This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Alternative Names | Coding region determinant-binding protein antibody CRD BP antibody CRD-BP antibody CRDBP antibody IF2B1 antibody IF2B1_HUMAN antibody IGF II mRNA binding protein 1 antibody IGF-II mRNA-binding protein 1 antibody IGF2 mRNA binding protein 1 antibody IGF2 mRNA-binding protein 1 antibody Coding region determinant-binding protein antibody CRD BP antibody CRD-BP antibody CRDBP antibody IF2B1 antibody IF2B1_HUMAN antibody IGF II mRNA binding protein 1 antibody IGF-II mRNA-binding protein 1 antibody IGF2 mRNA binding protein 1 antibody IGF2 mRNA-binding protein 1 antibody IGF2BP1 antibody IMP 1 antibody IMP-1 antibody IMP1 antibody Insulin like growth factor 2 mRNA binding protein 1 antibody Insulin-like growth factor 2 mRNA-binding protein 1 antibody VICKZ family member 1 antibody VICKZ1 antibody ZBP 1 antibody ZBP-1 antibody ZBP1 antibody Zip code binding protein 1 antibody Zip code-binding protein 1 antibody Zipcode binding protein 1 antibody Zipcode-binding protein 1 antibody |
| Molecular Weight(MW) | 63kDa |
| Cellular Localization | Nucleus, Cytoplasm. |
WB
Western blot analysis of IGF2BP1 on different lysates with Rabbit anti-IGF2BP1 antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: Caco-2 cell lysate, Lane 3: K-562 cell lysate, Lane 4: 293T cell lysate, Lane 5: MCF7 cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Caco-2 (positive) and MCF7 (negative) labeling IGF2BP1 with Rabbit anti-IGF2BP1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGF2BP1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of MCF7 (left, negative) and Caco-2 (right, positive) cells labeling IGF2BP1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 ICC/IF:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
