Application
Flow cytometric analysis of widr cells using IGFBP6 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with IGFBP6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of IGFBP6 Antibody in CEM cell line lysates (35ug/lane)| Product Name | IGFBP6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-like growth factor-binding protein 6, IBP-6, IGF-binding protein 6, IGFBP-6, IGFBP6, IBP6 |
| Product description | IGFBP6 (IGF binding protein 6) is a member of the IGFBP family that regulates the actions of IGFs. It acts extracellularly in an autocrine/paracrine manner. IGFBP6 binds preferentially to IGF-II. IGFBP6 has been shown to inhibit the growth of tumour cells and to promote apotosis.1) Guey,L.T., et.al., Eur. Urol. (2009) |
| Immunogen | This IGFBP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-200 amino acids from the C-terminal region of human IGFBP6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IGFBP6 |
|---|---|
| Alternative Names | Insulin-like growth factor-binding protein 6 IBP-6 IGF-binding protein 6 IGFBP-6 IGFBP6 IBP6 |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of widr cells using IGFBP6 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with IGFBP6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of IGFBP6 Antibody in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
