Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).| Product Name | IkB a (phospho Ser32 / Ser36) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKBA, MAD-3, NFKBI, ADDA, I-kappa-B-alpha, IKBA, NF-kappaB inhibitor alpha, NFKBIA |
| Product description | Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.1) Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab, N. H. et al. (1999) Proc . Natl . Acad. Sci. USA 96, 13777-13782. |
| Immunogen | IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α. |
| Modification | p-Ser32 / Ser36 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIA |
|---|---|
| Alternative Names | IKBA MAD-3 NFKBI ADDA I-kappa-B-alpha IKBA NF-kappaB inhibitor alpha NFKBIA |
| Molecular Weight(MW) | 39 kDa |
| Tissue Specificity | This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36. |
| Entrez Gene | 4792 |
|---|---|
| Protein Accession | NP_065390.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
