Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (phospho-Tyr42).| Product Name | IkB a (phospho Tyr42) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKBA, MAD-3, NFKBI, CDC2, CDC28A, CDKN1, P34CDC2, IKBA, NFKBIA, |
| Product description | Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.1) Beraud C, et al. (1999) Proc Natl Acad Sci U S A 96 (2): 429-434. Sundstrom S, et al. (2005) J Virol 79 (4): 2230-2239. Liu L, et al. (1998) Mol Cell Biol 18 (7): 4221-4234. Shrivastava A, et al. (1998) J Virol 72 (12): 9722-9728. |
| Immunogen | IkB-a (Phospho-Tyr42) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 42 (E-E-Y (p) -E-Q) derived from Human IkappaB-α. |
| Modification | p-Tyr42 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIA |
|---|---|
| Alternative Names | IKBA MAD-3 NFKBI CDC2 CDC28A CDKN1 P34CDC2 IKBA NFKBIA |
| Molecular Weight(MW) | 39 kDa |
| Tissue Specificity | This antibody detects endogenous levels of IkappaB-α only when phosphorylated at tyrosine 42. |
| Entrez Gene | 4792 |
|---|---|
| Protein Accession | NP_065390.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (phospho-Tyr42).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
