Application
Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.Application
Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.| Product Name | IKK gamma Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKK gamma Antibody: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, ZC2HC9, IKK-gamma, NF-kappa-B essential modulator |
| Product description | IKK gamma Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IκB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.1) Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-γ is an essential regulatory subunit of the IκB kinase complex. Nature 1998;395(6699):297-300 |
| Immunogen | IKK gamma antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK gamma. The immunogen is located within the last 50 amino acids of IKK gamma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| IKK gamma antibody can be used for detection of IKK gamma by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKG |
|---|---|
| Alternative Names | IKK gamma Antibody: IP IP1 IP2 FIP3 IPD2 NEMO FIP-3 Fip3p AMCBX1 ZC2HC9 IKK-gamma NF-kappa-B essential modulator |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | IKK gamma has no cross response to IKK alpha or IKK beta. |
Application
Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.
Application
Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IKK gamma antibody can be used for detection of IKK gamma by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IKK gamma antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IKK gamma Antibody is supplied in PBS containing 0.02% sodium azide. |
