Application
Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-IKK-gamma (Ser85) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | IKK-γ (phospho Ser85) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FIP-3, FIP3, I-kappa-B kinase gamma, IKK-gamma, IKKAP1 |
| Product description | Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.1) Niu J, Shi Y, Iwai K, Wu ZH (2011) EMBO J 30, 3741-53. Wu ZH, et al. (2010)Mol Cell 40, 75-86. Palkowitsch L, Leidner J, Ghosh S, Marienfeld RB (2008)J Biol Chem 283, 76-86. |
| Immunogen | IKK-γ (phospho Ser85) antibody was raised against a peptide sequence around phosphorylation site of serine 85 (Q-A-S (p)-Q-R) derived from Human IKK-gamma. |
| Modification | p-Ser85 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKG |
|---|---|
| Alternative Names | FIP-3 FIP3 I-kappa-B kinase gamma IKK-gamma IKKAP1 |
| Molecular Weight(MW) | 48 kDa |
| Tissue Specificity | The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85. |
| Entrez Gene | 8517 |
|---|---|
| Protein Accession | NP_001093326.2 |
Application
Western blot analysis of extracts from HepG2 cells treated with Anisomycin using Phospho-IKK-gamma (Ser85) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
