Application
Immunohistochemistry of IL-15 in spleen tissue with IL-15 antibody at 2.5 μg/ml.Application
Western blot analysis of IL-15 in rat spleen tissue lysate with IL-15 antibody at (A) 1 and (B) 2 μg/mL| Product Name | IL-15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-15 Antibody: IL-15, Interleukin-15, IL-15 |
| Product description | IL-15 Antibody: Interleukin 15 (IL-15) is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis.1) Bodnar A, Nizsaloczki E, Mocsar G, et al. A biophysical approach to IL-2 and IL-15 receptor function: localization, conformation and interactions. Immunol. Lett. 2008; 116:117-25. |
| Immunogen | Rabbit polyclonal IL-15 antibody was raised against a 13 amino acid peptide near the center of human IL-15. The immunogen is located within amino acids 50 - 100 of IL-15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IL-15 antibody can be used for detection of IL-15 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL15 |
|---|---|
| Alternative Names | IL-15 Antibody: IL-15 Interleukin-15 IL-15 |
| Molecular Weight(MW) | 18 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Guinea pig: (100%) |
| Tissue Specificity | At least two isoforms of IL-15 are known to exist. |
Application
Immunohistochemistry of IL-15 in spleen tissue with IL-15 antibody at 2.5 μg/ml.
Application
Western blot analysis of IL-15 in rat spleen tissue lysate with IL-15 antibody at (A) 1 and (B) 2 μg/mL| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
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| Application Notes | IL-15 antibody can be used for detection of IL-15 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | IL-15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IL-15 Antibody is supplied in PBS containing 0.02% sodium azide. |
