| Product Name | IL-15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin 15Interleukin-15IL-15, IL-15 |
| Product description | IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. |
| Immunogen | The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-15 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 250 pg/mL of recombinant human IL-15 when used with our biotinylated antigen affinity purified anti-human IL-15 (XP-5169Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL (non-reducing) or at a concentration of 2.0-4.0 μg/mL (reducing). Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL15 |
|---|---|
| Alternative Names | Interleukin 15Interleukin-15IL-15 IL-15 |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 250 pg/mL of recombinant human IL-15 when used with our biotinylated antigen affinity purified anti-human IL-15 (XP-5169Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL (non-reducing) or at a concentration of 2.0-4.0 μg/mL (reducing). Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-15 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
